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For research and educational purposes only. Not intended for human consumption.

Wolverine Stack

Well Researched
Updated Dec 2025

BPC-157 + TB-500 | Tissue Repair & Recovery Protocol

InjectableOral
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BPC-157: 250-500mcg + TB-500: 2-2.5mg
BPC-157: 1-2x daily, TB-500: 2x weekly
Injectable
Subcutaneous near injury site or abdomen
4-8 weeks
Typical duration
2-8°C
Refrigerated

Overview

What is Wolverine Stack?

The Wolverine Stack combines BPC-157 (Body Protection Compound-157) and TB-500 (Thymosin Beta-4 fragment) for enhanced tissue repair and recovery. Named after the Marvel character known for regenerative abilities, this combination targets complementary healing pathways: BPC-157 increases actin production and promotes angiogenesis via nitric oxide modulation, while TB-500 binds actin to facilitate cell migration and reduce scarring. A 2021 clinical study showed 87.5% of patients experienced knee pain relief. Banned by WADA

Key Benefits

Combines complementary healing mechanisms: BPC-157 promotes angiogenesis and reduces inflammation while TB-500 enhances cell migration and reduces scarring. Clinical study showed 87.5% improvement in knee pain patients.

Mechanism of Action

BPC-157 increases actin production at gene level and modulates nitric oxide for vascular effects. TB-500 is an actin-binding protein that sequesters actin for cell migration. Together they enhance fibroblast and immune cell movement to injury sites through synergistic actin regulation.

Molecular Information

Variable (combination)
Weight
0
amino acids
Peptide combination stack
Type
Amino Acid Sequence:
BPC-157: GEPPPGKPADDAGLV + TB-500: Full 43-aa sequence
* Combined protocol using two separate peptides with synergistic mechanisms

Pharmacokinetics

Peak
BPC-157: <30 min; TB-500: 2-4 hours
Half-life
BPC-157: <30 min; TB-500: longer
Cleared
Variable
100%50%0%0h6h12h18h24h
Peak
Half-life
Cleared
Preclinical data

Research Indications

Tendon & Ligament Healing

Both peptides accelerate tendon and ligament repair. BPC-157 improved Achilles tendon healing in animal models.

Muscle Injury Recovery

BPC-157 enhanced muscle regeneration. TB-500 promotes cell migration for muscle repair.

Joint Pain & Cartilage

Lee & Padgett study showed 87.5% of knee pain patients improved with BPC-157/TB4 combination.

Research Protocols

Disclaimer: Research protocol only. Both peptides are banned by WADA. Contraindicated with cancer history.

GoalDoseFrequencyRoute
General RecoveryBPC-157: 250mcg 2x/day + TB-500: 2mg 2x/weekBPC daily, TB-500 Mon/ThuSubcutaneous
Intensive Injury RecoveryBPC-157: 500mcg 2x/day + TB-500: 2.5mg 2x/weekBPC daily, TB-500 twice weeklySubQ near injury site
Maintenance/PreventionBPC-157: 250mcg 1x/day + TB-500: 2mg 1x/weekBPC daily, TB-500 weeklySubcutaneous

Timing: BPC-157 has short half-life so twice daily dosing maintains levels. TB-500 allows twice weekly dosing. Can inject same time but use separate sites.

Peptide Interactions

How to Reconstitute

Important: Always use bacteriostatic water (BAC). Sterile technique is essential.

1

Reconstitute each peptide separately

2

BPC-157: Add 2mL BAC water to 5mg vial = 250mcg per 0.1mL

3

TB-500: Add 2mL BAC water to 5mg vial = 250mcg per 0.1mL

4

Inject slowly down vial wall, swirl gently - never shake

5

Do NOT mix peptides in same syringe unless pre-made blend

6

Use separate injection sites if administering same day

Dosing Calculator

Calculate your injection volume with visual dosing guide

FINAL CONCENTRATION
2.50mg/mL
Each milliliter contains 2.50 mg of peptide
VISUAL REFERENCE (RESEARCH USE ONLY)

To obtain 250 mcg from this solution:

Draw 0.10 mL=10 units

(1 mL = 100 units on any insulin syringe)

0102030405060708090100
00.10.20.30.40.50.60.70.80.91.0
0.10 mL
10 units

Draw to this mark for 250 mcg

This calculator is for research purposes only. Always verify calculations and consult protocols.

Quality Indicators

White lyophilized powder

Both vials should contain white or off-white fluffy powder cake.

Clear after reconstitution

Both solutions should be crystal clear with no particles.

Certificate of Analysis

HPLC purity testing (>98%) and mass spec verification for both.

Collapsed or discolored

Yellowed or stuck powder may be heat-degraded - do not use.

What to Expect

  • Week 1-2: Reduction in acute inflammation and pain at injury sites
  • Week 2-4: Improved recovery between workouts, reduced soreness
  • Week 4-6: Significant improvement in chronic injuries, joint comfort
  • Week 6-8: Optimal healing effects, structural improvements
  • Post-cycle: Benefits may persist as healed tissue maintains integrity

Side Effects & Safety

Side Effects

  • Not FDA approved - research chemical only
  • Contraindicated with ANY history of cancer
  • Banned by WADA and major sports organizations
  • Limited human safety data - most research preclinical
  • Do not combine with chemotherapy
  • Monitor for unusual tissue growth

When to Stop

  • Any unusual growths or tissue changes
  • Signs of adverse effects
  • As directed by healthcare provider

References

3 Studies

BPC-157 Systematic Review (2025)

36 articles | Orthopedic focus | Preclinical improvements in muscle, tendon, ligament, fracture

Enhanced GH receptor expression and angiogenesis with reduced inflammatory cytokines.

BPC-157 + TB4 Knee Pain Study (2021)

Human | 16 patients | Intra-articular | 87.5% experienced knee pain relief

First published study combining BPC-157 and TB4. BPC-157 alone: 91.6% improvement. Combination: 75% improvement.

View Study

Thymosin β4 Wound Healing Phase 2 (2012)

Human | Stasis and pressure ulcers | Accelerated healing by ~1 month

TB4 accelerated wound healing in patients who responded to treatment.

View Study

Quick Start Guide

Typical Dose
BPC-157: 250-500mcg + TB-500: 2-2.5mg
How Often
BPC: 1-2x daily, TB-500: 2x weekly
Where to Inject
SubQ near injury or abdomen/thigh
Timing
BPC: morning/evening; TB-500: any consistent days
Effects Timeline
Initial: 1-2 weeks; Significant: 4-6 weeks; Full: 8 weeks
Storage
Reconstituted: refrigerate 2-8°C, use within 3-4 weeks
Cycle Length
4-8 weeks
Break Between
4 weeks off recommended

Research Disclaimer

Wolverine Stack is sold for laboratory research purposes only and is not intended for human or animal consumption. The information provided on this page is compiled from published research, veterinary studies, and anecdotal reports for educational purposes. This content does not constitute medical advice, diagnosis, or treatment recommendations. Any research involving Wolverine Stack must comply with all applicable local, state, and federal regulations. BioInfinity Lab makes no claims regarding the safety or efficacy of Wolverine Stack for any purpose. Consult qualified professionals for any research applications.