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For research and educational purposes only. Not intended for human consumption.

VIP

Well Researched
Updated Dec 2025

Vasoactive Intestinal Peptide | Immune Regulation & Neuroprotection

InjectableNasal
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50-100mcg per spray
2-4x daily
Injectable
N/A - Intranasal spray
4-12 weeks or ongoing
Typical duration
2-8°C
Refrigerated

Overview

What is VIP?

Vasoactive Intestinal Peptide (VIP) is a 28-amino acid neuropeptide with potent anti-inflammatory, immunomodulatory, and neuroprotective properties. Acting through VPAC1 and VPAC2 receptors, VIP regulates immune function, vascular tone, circadian rhythms, and neurological health. The injectable form (Aviptadil) received FDA Fast Track designation for COVID-19 ARDS.

Key Benefits

Immune regulation and reduced inflammation, neuroprotection and circadian rhythm support, respiratory health improvement, direct CNS delivery bypassing BBB, correction of CIRS inflammatory markers.

Mechanism of Action

VIP binds to VPAC1 and VPAC2 receptors coupled to Gαs proteins, activating adenylate cyclase and increasing cAMP/PKA signaling. This leads to anti-inflammatory effects through inhibition of pro-inflammatory cytokines (IL-6, TNF-α) and modulation of immune responses.

Molecular Information

3,326.8 Da
Weight
28
amino acids
Linear neuropeptide
Type
Amino Acid Sequence:
His-Ser-Asp-Ala-Val-Phe-Thr-Asp-Asn-Tyr-Thr-Arg-Leu-Arg-Lys-Gln-Met-Ala-Val-Lys-Lys-Tyr-Leu-Asn-Ser-Ile-Leu-Asn-NH2
* C-terminal amidation for stability

Pharmacokinetics

Peak
15 min
Half-life
30 min
Cleared
~2.5 hrs
100%50%0%0h6h12h18h24h
Peak
Half-life
Cleared
Youssef et al. 2022

Research Indications

CIRS (Chronic Inflammatory Response Syndrome)

VIP nasal spray is the final step in the Shoemaker Protocol for CIRS from mold/biotoxin exposure. Corrects inflammatory markers refractory to other therapies.

Autoimmune Conditions

Research shows VIP downregulates inflammatory and autoimmune disease components.

Respiratory Inflammation

VIP has bronchodilatory and anti-inflammatory effects in airways.

Research Protocols

Disclaimer: VIP nasal spray is compounded off-label and not FDA-approved. For CIRS, complete Shoemaker Protocol steps 1-11 before starting VIP. Consult a specialist.

GoalDoseFrequencyRoute
CIRS Protocol (Shoemaker)50mcg per spray, 4-8 sprays/day4x dailyIntranasal
General Immune Support50-100mcg2-4x dailyIntranasal
Starting/Sensitive Patients50mcg2x dailyIntranasal
Advanced/Maintenance100-200mcg2-4x dailyIntranasal

Timing: Multiple daily doses maintain therapeutic levels due to short half-life. Morning dosing aligns with natural circadian VIP rhythms.

Peptide Interactions

How to Reconstitute

Important: Always use bacteriostatic water (BAC). Sterile technique is essential.

1

VIP nasal spray is typically supplied pre-formulated by compounding pharmacies

2

Store in refrigerator at 2-8°C

3

Clear nasal passages before administration

4

Prime spray bottle before first use (2-3 pumps)

5

Insert nozzle into nostril, aim slightly outward

6

Spray while gently inhaling

7

Alternate nostrils between doses

8

Do not blow nose immediately after spraying

Dosing Calculator

Calculate your injection volume with visual dosing guide

FINAL CONCENTRATION
2.50mg/mL
Each milliliter contains 2.50 mg of peptide
VISUAL REFERENCE (RESEARCH USE ONLY)

To obtain 250 mcg from this solution:

Draw 0.10 mL=10 units

(1 mL = 100 units on any insulin syringe)

0102030405060708090100
00.10.20.30.40.50.60.70.80.91.0
0.10 mL
10 units

Draw to this mark for 250 mcg

This calculator is for research purposes only. Always verify calculations and consult protocols.

Quality Indicators

Clear solution

Compounded VIP nasal spray should be clear and colorless.

Proper spray function

Should deliver consistent, fine mist.

Licensed compounding pharmacy

Source from accredited pharmacy with COA.

Temperature during shipping

VIP is temperature-sensitive. Ensure cold chain.

Cloudiness or particles

Indicates degradation - do not use.

What to Expect

  • Days 1-7: May experience mild nasal irritation initially
  • Week 1-2: Potential improvement in energy and sleep quality
  • Week 2-4: Reduction in inflammatory symptoms
  • Week 4-8: Progressive improvement in CIRS markers if applicable
  • Month 2+: Continued correction of inflammatory parameters

Side Effects & Safety

Side Effects

  • First dose should be administered under medical supervision with lab monitoring
  • Pre-VIP labs: TGF-β1, Lipase (baseline)
  • Stop immediately if abdominal pain develops or lipase elevates
  • Not FDA-approved as nasal spray - compounded off-label
  • Monitor blood pressure - VIP can cause vasodilation
  • Not recommended during pregnancy or breastfeeding

When to Stop

  • Abdominal pain
  • Elevated lipase
  • Significant blood pressure changes
  • Allergic reactions
  • As directed by specialist

References

3 Studies

IV Aviptadil in COVID-19 Respiratory Failure (2022)

Human | 196 patients | IV infusion | 60 days | 2-fold increased survival odds

Multicenter RCT showing Aviptadil increased 60-day survival odds 2-fold in critical COVID-19.

VIP Corrects CIRS (2013)

Human | CIRS patients | Nasal spray | Multiple months | Correction of inflammatory markers

VIP nasal spray corrects CIRS from water-damaged buildings, improving proteomics and transcriptomics.

IUPHAR VIP/PACAP Review (2012)

Review | VPAC1/VPAC2/PAC1 receptors | Comprehensive pharmacology

Definitive review of VIP receptor pharmacology and therapeutic potential.

Quick Start Guide

Typical Dose
50-100mcg per spray
How Often
2-4x daily
Where to Inject
N/A - Intranasal spray
Timing
Morning and evening, or spread throughout day
Effects Timeline
Days to weeks for symptom improvement, months for full CIRS correction
Storage
Refrigerate 2-8°C
Cycle Length
4-12 weeks or ongoing based on condition
Break Between
May use continuously under medical supervision

Research Disclaimer

VIP is sold for laboratory research purposes only and is not intended for human or animal consumption. The information provided on this page is compiled from published research, veterinary studies, and anecdotal reports for educational purposes. This content does not constitute medical advice, diagnosis, or treatment recommendations. Any research involving VIP must comply with all applicable local, state, and federal regulations. BioInfinity Lab makes no claims regarding the safety or efficacy of VIP for any purpose. Consult qualified professionals for any research applications.