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For research and educational purposes only. Not intended for human consumption.

Thymosin Beta-4

Well Researched
Updated Dec 2025

43-Amino Acid Regenerative Peptide | Tissue Repair & Healing

InjectableTopical
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2-6mg daily
Daily or weekly
Injectable
Subcutaneous: abdomen
2-6 or 8-12 weeks
Typical duration
2-8°C
Refrigerated

Overview

What is Thymosin Beta-4?

Thymosin Beta-4 (Tβ4) is a naturally occurring 43-amino acid peptide that plays a crucial role in tissue repair, wound healing, and cellular regeneration. It promotes angiogenesis, reduces inflammation, and supports the migration and differentiation of various cell types involved in healing processes. Banned by WADA

Key Benefits

Systemic regenerative effects, proven clinical efficacy, optimal bioavailability for tissue repair.

Mechanism of Action

Direct systemic delivery provides optimal bioavailability and distribution to target tissues for comprehensive regenerative effects. Promotes actin sequestration, angiogenesis, and stem cell recruitment.

Molecular Information

4,963.44 Da
Weight
43
amino acids
Thymosin peptide
Type
Amino Acid Sequence:
Ac-Ser-Asp-Lys-Pro-Asp-Met-Ala-Glu-Ile-Glu-Lys-Phe-Asp-Lys-Ser-Lys-Leu-Lys-Lys-Thr-Glu-Thr-Gln-Glu-Lys-Asn-Pro-Leu-Pro-Ser-Lys-Glu-Thr-Ile-Glu-Gln-Glu-Lys-Gln-Ala-Gly-Glu-Ser
* N-terminal acetylation, naturally occurring in thymus. TB-500 is the active region (17-23) of this full sequence.

Pharmacokinetics

Peak
2 hrs
Half-life
2-3 hrs
Cleared
~12 hrs
100%50%0%0h6h12h18h24h
Peak
Half-life
Cleared
Phase I clinical data

Research Indications

Wound Healing Acceleration

Clinical trials show 67% complete healing vs 25% placebo in pressure ulcers.

Post-Surgical Recovery

Enhanced tissue regeneration and reduced healing time in surgical wounds.

Soft Tissue Injury Repair

Accelerated recovery in muscle, tendon, and ligament injuries.

Research Protocols

Disclaimer: Thymosin Beta-4 is the full-length parent peptide of TB-500. These protocols are based on clinical research. Consult a healthcare provider before use.

GoalDoseFrequencyRoute
Acute Wound Healing1.6mgDailySubcutaneous injection
Cardiac Protection42mgSingle doseIV bolus within 6 hours of MI
Chronic Tissue Repair6mgTwice weeklySubcutaneous injection
Neurological Recovery30mgThree times over 72 hoursIV infusion
General Regeneration2-5mgDaily or every other daySubcutaneous injection

Timing: Most effective when started early in the injury/healing process. For cardiac protection, must be given within 6 hours of symptom onset.

Peptide Interactions

How to Reconstitute

Important: Always use bacteriostatic water (BAC). Sterile technique is essential.

1

Allow lyophilized powder to reach room temperature before reconstitution

2

Add bacteriostatic water slowly to the vial wall, avoiding direct contact with powder

3

Gently swirl (do not shake vigorously) until completely dissolved

4

Inspect solution for clarity - should be clear and colorless

5

Store reconstituted solution at 2-8°C and use within 14 days

6

For injection, use proper aseptic technique and rotate injection sites

Dosing Calculator

Calculate your injection volume with visual dosing guide

FINAL CONCENTRATION
2.50mg/mL
Each milliliter contains 2.50 mg of peptide
VISUAL REFERENCE (RESEARCH USE ONLY)

To obtain 250 mcg from this solution:

Draw 0.10 mL=10 units

(1 mL = 100 units on any insulin syringe)

0102030405060708090100
00.10.20.30.40.50.60.70.80.91.0
0.10 mL
10 units

Draw to this mark for 250 mcg

This calculator is for research purposes only. Always verify calculations and consult protocols.

Quality Indicators

Clear, colorless solution

Reconstituted Thymosin β4 should be clear without particles.

Proper sterile packaging

Lyophilized powder in sterile vials with intact seals.

Certificate of analysis

Third-party testing showing >98% purity.

Appropriate molecular weight

HPLC/MS confirmation of 4,963 Da.

Cloudy or discolored solution

Indicates degradation or contamination.

Crystallization after reconstitution

Suggests improper storage or expired product.

What to Expect

  • Week 1-2: Initial tissue response, possible mild injection site reactions
  • Week 2-4: Accelerated healing becomes apparent, reduced inflammation
  • Week 4-8: Significant tissue regeneration and functional improvement
  • Week 8-12: Sustained benefits and near-complete healing in responsive conditions

Side Effects & Safety

Side Effects

  • Monitor injection sites for local reactions or inflammation
  • Use sterile technique to prevent infection at injection sites
  • Start with lower doses to assess individual tolerance
  • Discontinue if signs of allergic reaction or unusual side effects

When to Stop

  • Signs of allergic reaction
  • Severe injection site reactions
  • Unexpected symptoms
  • As directed by healthcare provider

References

3 Studies

Cardiac Protection Pilot Study (2016)

Human | EPC pre-treatment with Tβ4 | Single transplantation | Improved cardiac function

First human study demonstrating potential clinical benefits in repairing damaged cardiac tissue.

Phase II Dry Eye Clinical Trial (2015)

Human | 0.1% topical drops | 28 days | Significant symptom improvement

Randomized study in 72 subjects showing significant positive effects on ocular discomfort.

Phase I Human Safety Study (2010)

Human | 42-1260 mg IV | Single and multiple doses | Excellent safety profile

First-in-human study establishing safety and pharmacokinetics of IV Thymosin β4.

Quick Start Guide

Typical Dose
2-6mg daily
How Often
Once daily or every other day
Where to Inject
Subcutaneous: abdomen
Timing
Can be given any time of day; for acute conditions, start as soon as possible
Effects Timeline
Initial: 24-48 hours; Tissue repair: 1-2 weeks; Full regeneration: 4-12 weeks
Storage
Lyophilized: room temperature; Reconstituted: 2-8°C for up to 14 days
Cycle Length
Acute: 2-6 weeks; Chronic: 8-12 weeks
Break Between
2-4 weeks between treatment cycles

Research Disclaimer

Thymosin Beta-4 is sold for laboratory research purposes only and is not intended for human or animal consumption. The information provided on this page is compiled from published research, veterinary studies, and anecdotal reports for educational purposes. This content does not constitute medical advice, diagnosis, or treatment recommendations. Any research involving Thymosin Beta-4 must comply with all applicable local, state, and federal regulations. BioInfinity Lab makes no claims regarding the safety or efficacy of Thymosin Beta-4 for any purpose. Consult qualified professionals for any research applications.