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For research and educational purposes only. Not intended for human consumption.

SS-31 (Elamipretide)

Extensively Studied
Updated Dec 2025

Mitochondrial-Targeted Peptide | Cardiolipin Protector

InjectableTopical
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5-40mg
Once daily
Injectable
Subcutaneous: Abdomen
4-12 weeks
Typical duration
2-8°C
Refrigerated

Overview

What is SS-31 (Elamipretide)?

SS-31, also known as Elamipretide, achieved a major milestone in September 2025 when the FDA granted accelerated approval for Forzinity (elamipretide injection) as the FIRST-EVER mitochondria-targeted therapeutic, approved to improve muscle strength in adult and pediatric patients with Barth syndrome weighing at least 30kg. This represents a breakthrough for all mitochondrial diseases.

Key Benefits

FIRST FDA-approved mitochondria-targeted therapy (September 2025). Directly protects cardiolipin in inner mitochondrial membrane, improves ATP production, enhances electron transport chain function, reduces oxidative stress. High safety profile across multiple clinical trials.

Mechanism of Action

Selectively binds to cardiolipin, a phospholipid essential for mitochondrial structure and function, normalizing the inner mitochondrial membrane structure. This improves ATP production, enhances electron transport chain function, and reduces harmful oxidative stress at the source.

Molecular Information

639.8 Da
Weight
4
amino acids
Aromatic-cationic tetrapeptide
Type
Amino Acid Sequence:
D-Arg-2',6'-dimethyltyrosine-Lys-Phe-NH2
* Mitochondrial-targeting peptide that binds cardiolipin in inner mitochondrial membrane

Pharmacokinetics

Peak
30 min
Half-life
4 hrs
Cleared
~20 hrs
100%50%0%0h6h12h18h24h
Peak
Half-life
Cleared
Stealth BioTherapeutics trials

Research Indications

Enhanced ATP Production

Improves mitochondrial efficiency and cellular energy generation.

Oxidative Stress Reduction

Protects against reactive oxygen species at the source.

Mitochondrial Disease Support

FDA-approved for Barth syndrome. Shows promise for other mitochondrial disorders.

Research Protocols

Disclaimer: SS-31 (Elamipretide) is FDA-approved as FORZINITY for Barth syndrome. Other uses are investigational. Consult a healthcare provider.

GoalDoseFrequencyRoute
General Mitochondrial Support5-10mgOnce dailySubcutaneous
Athletic Performance10-20mgOnce daily pre-workoutSubcutaneous
Clinical Protocols40mgOnce dailySubcutaneous or IV
Acute Cardioprotection0.25mg/kg/hrContinuous infusionIntravenous

Timing: Morning dosing recommended to align with natural circadian rhythms of mitochondrial function. Pre-workout timing for athletic applications.

Peptide Interactions

How to Reconstitute

Important: Always use bacteriostatic water (BAC). Sterile technique is essential.

1

Clean vial top with alcohol pad

2

Add appropriate amount of bacteriostatic water (typically 1-2mL per 10mg)

3

Gently swirl to dissolve - SS-31 dissolves readily

4

Solution should be clear and colorless

5

For IV use, may require sterile filtration

6

Label with concentration and date

7

Store in refrigerator immediately

Dosing Calculator

Calculate your injection volume with visual dosing guide

FINAL CONCENTRATION
2.50mg/mL
Each milliliter contains 2.50 mg of peptide
VISUAL REFERENCE (RESEARCH USE ONLY)

To obtain 250 mcg from this solution:

Draw 0.10 mL=10 units

(1 mL = 100 units on any insulin syringe)

0102030405060708090100
00.10.20.30.40.50.60.70.80.91.0
0.10 mL
10 units

Draw to this mark for 250 mcg

This calculator is for research purposes only. Always verify calculations and consult protocols.

Quality Indicators

Pharmaceutical Grade

SS-31 requires high purity due to clinical use - look for >98% purity.

Clear Solution

Should dissolve completely into clear, colorless solution.

COA Available

Certificate of analysis essential due to clinical applications.

Light Sensitive

Protect from light exposure - use amber vials if available.

pH Considerations

Solution pH should be near physiological (6.5-7.5).

Discoloration

Any yellow or brown color indicates degradation.

What to Expect

  • Day 1-3: Subtle energy improvements, reduced fatigue
  • Week 1-2: Better exercise endurance, faster recovery
  • Week 2-4: Improved stamina, clearer thinking, better sleep quality
  • Week 4-8: Significant improvements in exercise capacity
  • Week 8-12: Sustained energy improvements, potential biomarker changes

Side Effects & Safety

Side Effects

  • Excellent safety profile in clinical trials
  • No significant side effects reported at therapeutic doses
  • May cause mild injection site reactions
  • Monitor for allergic reactions (very rare)
  • Safe for long-term use based on current data
  • No known drug interactions

When to Stop

  • Allergic reactions
  • Severe injection site reactions
  • Unexpected symptoms
  • As directed by physician
  • Before certain medical procedures

References

5 Studies

FDA Accelerated Approval (September 2025)

First-ever mitochondria-targeted therapy | Forzinity approved for Barth syndrome

September 2025: FDA granted accelerated approval as the FIRST mitochondrial-targeted therapeutic approved. Improves muscle strength in Barth syndrome patients ≥30kg. Landmark achievement for all mitochondrial disease research.

Johns Hopkins Barth Syndrome Trial (2021)

Human | Various doses | Energy production | Improved mitochondrial protein complex assembly

Clinical trial showed elamipretide improved energy production and assembly of important protein complexes in mitochondria. Published in Journal of Biological Chemistry.

Renal Artery Stenosis Phase 2a (2017)

Human | 14 patients | Severe atherosclerotic RAS | Increased blood flow and kidney function

Phase 2a trial showed increased blood flow to kidneys and improved kidney function in patients with severe atherosclerotic renal artery stenosis.

Quick Start Guide

Typical Dose
10-40mg depending on application
How Often
Once daily, preferably morning
Where to Inject
Subcutaneous: Abdomen
Timing
Morning for general use, pre-workout for performance
Effects Timeline
Energy improvements within days, exercise capacity 2-4 weeks, clinical benefits 4-12 weeks
Storage
Refrigerate after reconstitution, protect from light, use within 30 days
Cycle Length
4-12 weeks continuous use
Break Between
No mandatory break required - can be used continuously

Research Disclaimer

SS-31 (Elamipretide) is sold for laboratory research purposes only and is not intended for human or animal consumption. The information provided on this page is compiled from published research, veterinary studies, and anecdotal reports for educational purposes. This content does not constitute medical advice, diagnosis, or treatment recommendations. Any research involving SS-31 (Elamipretide) must comply with all applicable local, state, and federal regulations. BioInfinity Lab makes no claims regarding the safety or efficacy of SS-31 (Elamipretide) for any purpose. Consult qualified professionals for any research applications.