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For research and educational purposes only. Not intended for human consumption.

Semax

Well Researched
Updated Dec 2025

Synthetic ACTH Analog | Cognitive Enhancement Peptide

NasalInjectable
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300-1000mcg
1-3x daily
Nasal
Nasal spray - alternate nostrils
2-4 weeks on, 1-2 weeks off
Typical duration
2-8°C
Refrigerated

Overview

What is Semax?

Semax is a synthetic heptapeptide derived from adrenocorticotropic hormone (ACTH) fragment 4-10, extensively studied for its nootropic, neuroprotective, and neurorestorative properties. Originally developed in Russia for stroke recovery, this brain-targeted peptide rapidly increases BDNF levels via CREB pathway and modulates dopaminergic and serotonergic systems. Recent 2024-2025 research demonstrates efficacy in Alzheimer's disease models, reducing amyloid inclusions and improving cognitive function, with emerging applications in ADHD and neurodegenerative conditions.

Key Benefits

Rapid brain delivery, extensive clinical research, easy self-administration, excellent bioavailability to brain regions, demonstrated amyloid-β fibril inhibition, enhanced attention and impulse control, and BDNF upregulation via CREB transcription factor.

Mechanism of Action

Direct transport through olfactory epithelium and trigeminal nerves, bypassing blood-brain barrier for enhanced CNS penetration. Enhances neurotrophin transcription (BDNF via CREB), modulates dopaminergic/noradrenergic systems in prefrontal cortex, inhibits Aβ fibril formation, and targets μ-opioid receptor (Oprm1) for neuroprotection.

Molecular Information

813.93 Da
Weight
7
amino acids
ACTH fragment analog
Type
Amino Acid Sequence:
Met-Glu-His-Phe-Pro-Gly-Pro
* Synthetic analog of ACTH(4-10) with nootropic properties

Pharmacokinetics

Peak
~20 min (intranasal)
Half-life
1-2 hrs (plasma)
Cleared
~4-6 hrs
100%50%0%0h6h12h18h24h
Peak
Half-life
Cleared
Russian clinical data - effects persist 20-24 hrs

Research Indications

Memory Enhancement

Improved short-term and working memory performance in fatigued individuals and healthy subjects.

Attention and Focus

Enhanced sustained attention during demanding cognitive tasks and prolonged work periods.

Learning Acceleration

Faster acquisition of new information and improved retention in educational settings.

Research Protocols

Disclaimer: Semax is approved in Russia for various neurological conditions. These protocols are for research purposes. Consult a healthcare provider before use.

GoalDoseFrequencyRoute
Cognitive enhancement (healthy adults)300-600mcg1-2 times dailyIntranasal spray/drops
Learning and memory support600-900mcg2-3 times daily during study periodsIntranasal spray/drops
Recovery from brain injury900-1500mcg2-3 times dailyIntranasal (clinical supervision)
Anti-fatigue cognitive support400-800mcgSingle morning doseIntranasal spray

Timing: Morning administration optimizes cognitive benefits throughout the day while avoiding evening alertness that could impact sleep.

Peptide Interactions

How to Reconstitute

Important: Always use bacteriostatic water (BAC). Sterile technique is essential.

1

Semax nasal formulations come pre-mixed as 0.1% or 1% solutions

2

For research use, lyophilized powder can be reconstituted with sterile saline

3

Use preservative-free saline for nasal administration to avoid irritation

4

Calculate concentration based on desired mcg per spray or drop

5

Store reconstituted solution refrigerated and use within recommended timeframe

6

Always use sterile technique when preparing nasal formulations

Dosing Calculator

Calculate your injection volume with visual dosing guide

FINAL CONCENTRATION
2.50mg/mL
Each milliliter contains 2.50 mg of peptide
VISUAL REFERENCE (RESEARCH USE ONLY)

To obtain 250 mcg from this solution:

Draw 0.10 mL=10 units

(1 mL = 100 units on any insulin syringe)

0102030405060708090100
00.10.20.30.40.50.60.70.80.91.0
0.10 mL
10 units

Draw to this mark for 250 mcg

This calculator is for research purposes only. Always verify calculations and consult protocols.

Quality Indicators

Clear, Colorless Solution

Semax nasal solution should be transparent without particles or cloudiness.

Proper Concentration Labeling

Clearly marked as 0.1% or 1% Semax with accurate mcg per spray information.

Cold Chain Shipping

Nasal formulations should arrive with cold packs and require immediate refrigeration.

Sterile Packaging

Sealed nasal spray bottles or ampoules with sterility assurance.

Nasal Irritation Potential

Some mild nasal discomfort possible - discontinue if severe irritation occurs.

Crystallization or Precipitation

Any visible crystals or particles indicate degradation or contamination.

What to Expect

  • Days 1-3: Initial cognitive clarity and focus enhancement, possible mild nasal sensation
  • Week 1: Improved attention span and mental stamina during demanding tasks
  • Week 2-3: Enhanced memory consolidation and learning capacity, stable mood effects
  • Week 4: Peak cognitive benefits with improved stress resilience and mental flexibility
  • Post-cycle: Gradual return to baseline over 3-7 days, some benefits may persist

Side Effects & Safety

Side Effects

  • Start with lowest effective dose (300mcg) to assess individual response
  • Avoid concurrent use with other intranasal medications that cause vasoconstriction
  • Monitor for any signs of nasal irritation, bleeding, or persistent discomfort
  • Do not exceed 4-week continuous use periods without medical supervision
  • Potential to enhance effects of stimulants - reduce other stimulant doses accordingly

When to Stop

  • Persistent nasal irritation or bleeding
  • Severe headaches
  • Sleep disturbances
  • Mood changes
  • Consult healthcare provider

References

6 Studies

Stroke Recovery Clinical Trial

Human | 110 patients | Semax + conventional therapy | Ischemic stroke recovery

Patients recovering from ischemic stroke who received Semax showed increased BDNF levels correlated with early rehabilitation and accelerated restoration of brain functions.

fMRI Default Mode Network Study

Human | 24 participants | Single dose intranasal | Brain imaging analysis

Healthy middle-aged participants showed increased fMRI default mode network activity relative to placebo, indicating enhanced neural connectivity.

Alzheimer's Disease Model (2025)

APP/PS1 mice | Intranasal administration | Improved cognition, reduced amyloid

In Alzheimer's disease mouse models, Semax improved cognitive functions (open field, novel object recognition, Barnes maze tests) and significantly reduced amyloid inclusions in cortex and hippocampus.

Quick Start Guide

Typical Dose
300-600mcg per administration
How Often
1-2 times daily, preferably morning and early afternoon
Where to Inject
Nasal spray - alternate nostrils
Timing
Morning for cognitive enhancement, avoid evening use
Effects Timeline
Onset 15-30 minutes, peak 1-2 hours, duration 4-6 hours
Storage
Refrigerate at 2-8°C, do not freeze, protect from light
Cycle Length
2-4 weeks continuous use maximum
Break Between
1-2 weeks break between cycles

Research Disclaimer

Semax is sold for laboratory research purposes only and is not intended for human or animal consumption. The information provided on this page is compiled from published research, veterinary studies, and anecdotal reports for educational purposes. This content does not constitute medical advice, diagnosis, or treatment recommendations. Any research involving Semax must comply with all applicable local, state, and federal regulations. BioInfinity Lab makes no claims regarding the safety or efficacy of Semax for any purpose. Consult qualified professionals for any research applications.