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For research and educational purposes only. Not intended for human consumption.

Testagen

Limited Research
Updated Dec 2025

KEDG Tetrapeptide | Anterior Pituitary Bioregulator

InjectableOral
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100-300mcg
Once daily
Injectable
Subcutaneous: abdomen
8-12 weeks
Typical duration
-20°C
Refrigerated

Overview

What is Testagen?

Testagen (KEDG, Lys-Glu-Asp-Gly) is a synthetic tetrapeptide bioregulator developed by Professor Vladimir Khavinson. Derived from anterior pituitary gland extracts, Testagen is researched for effects on thyroid-stimulating hormone (TSH) release, thyroid function, and downstream testosterone regulation. It may cross cell and nuclear membranes to directly interact with DNA.

Key Benefits

Direct systemic absorption, precise dosing control, bypasses GI degradation, supports anterior pituitary function, potential thyroid and testosterone optimization through TSH modulation.

Mechanism of Action

Testagen (KEDG) crosses cell and nuclear membranes to interact with DNA in anterior pituitary cells, stimulating TSH release and subsequently influencing T3/T4 thyroid hormone production, which correlates with normalized testosterone levels.

Molecular Information

447.4 Da
Weight
4
amino acids
Bioregulatory tetrapeptide
Type
Amino Acid Sequence:
H-Lys-Glu-Asp-Gly-OH (KEDG)
* Synthetic analog based on anterior pituitary gland peptide extracts

Pharmacokinetics

Peak
15 minutes
Half-life
30 minutes
Cleared
~2.5 hours
100%50%0%0h6h12h18h24h
Peak
Half-life
Cleared
Khavinson et al.

Research Indications

Anterior Pituitary Support

May stimulate anterior pituitary function, enhancing TSH release.

Thyroid Function Optimization

Studies indicate Testagen may increase TSH, influencing T3/T4 production.

Testosterone Support (Indirect)

By optimizing thyroid function, may indirectly support testosterone levels.

Research Protocols

Disclaimer: No human clinical trials exist. Guidance from preclinical research. Contraindicated in thyroid cancer or hyperthyroidism.

GoalDoseFrequencyRoute
Starting/Titration (Weeks 1-2)100mcgOnce dailySubQ
Building (Weeks 3-4)150mcgOnce dailySubQ
Standard (Weeks 5-8)200mcgOnce dailySubQ
Maximum (Weeks 9-12)250-300mcgOnce dailySubQ

Timing: Morning administration preferred to align with natural circadian hormone rhythms.

Peptide Interactions

How to Reconstitute

Important: Always use bacteriostatic water (BAC). Sterile technique is essential.

1

Add 3.0mL BAC water to 20mg vial = 6.67mg/mL

2

Inject slowly down vial wall

3

Gently swirl - do NOT shake

4

Allow to sit if cloudiness, swirl until clear

5

Store reconstituted at 2-8°C

Dosing Calculator

Calculate your injection volume with visual dosing guide

FINAL CONCENTRATION
2.50mg/mL
Each milliliter contains 2.50 mg of peptide
VISUAL REFERENCE (RESEARCH USE ONLY)

To obtain 250 mcg from this solution:

Draw 0.10 mL=10 units

(1 mL = 100 units on any insulin syringe)

0102030405060708090100
00.10.20.30.40.50.60.70.80.91.0
0.10 mL
10 units

Draw to this mark for 250 mcg

This calculator is for research purposes only. Always verify calculations and consult protocols.

Quality Indicators

White lyophilized powder

White, fluffy powder cake without clumping.

Clear reconstituted solution

Crystal clear and colorless.

Limited research base

Most research from Russian bioregulator programs.

Discoloration

Yellow/brown indicates degradation.

What to Expect

  • Weeks 1-2: Establishing baseline, minimal effects
  • Weeks 3-4: Subtle improvements in energy and mood
  • Weeks 5-8: Thyroid function optimization, improved metabolic rate
  • Weeks 8-12: Full effects on thyroid-testosterone axis

Side Effects & Safety

Side Effects

  • No human clinical trials - preclinical guidance only
  • Contraindicated in thyroid cancer or hyperthyroidism
  • Monitor thyroid function if on thyroid medications
  • Start with lower dose to assess response
  • Not recommended during pregnancy

When to Stop

  • Thyroid abnormalities
  • Adverse effects
  • As directed by provider

References

2 Studies

Hypophysectomized Avian Model Study

Avian | Pituitary-removed birds | Thyroid assessment | Thyroid morphology changes

KEDG peptide associated with changes in thyroid gland morphology and follicle structure even without hypophyseal support.

Thyroid Function Without Hypophyseal Support

Preclinical | TSH modulation | Gene expression analysis

Testagen stimulates TSH release and T3/T4 production even without hypophyseal support.

Quick Start Guide

Typical Dose
100-300mcg
How Often
Once daily
Where to Inject
SubQ: abdomen
Timing
Morning preferred for circadian alignment
Effects Timeline
Weeks 4-8 for hormonal effects
Storage
Lyophilized -20°C, Reconstituted 2-8°C
Cycle Length
8-12 weeks
Break Between
4-6 months between cycles

Research Disclaimer

Testagen is sold for laboratory research purposes only and is not intended for human or animal consumption. The information provided on this page is compiled from published research, veterinary studies, and anecdotal reports for educational purposes. This content does not constitute medical advice, diagnosis, or treatment recommendations. Any research involving Testagen must comply with all applicable local, state, and federal regulations. BioInfinity Lab makes no claims regarding the safety or efficacy of Testagen for any purpose. Consult qualified professionals for any research applications.