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For research and educational purposes only. Not intended for human consumption.

NAD+

Extensively Studied
Updated Dec 2025

Essential Cellular Coenzyme | Anti-Aging Therapy

InjectableOralTopicalNasal
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100-1000mg
1-3x weekly
Injectable
IM: deltoid
4-12 weeks
Typical duration
2-8°C
Refrigerated

Overview

What is NAD+?

Nicotinamide Adenine Dinucleotide (NAD+) is a vital coenzyme found in every cell that plays a crucial role in energy production, DNA repair, and cellular metabolism. As NAD+ levels naturally decline with age, supplementation can support cellular health, boost energy, enhance cognitive function, and promote longevity.

Key Benefits

Highest bioavailability (100%), rapid cellular uptake, immediate energy enhancement, bypasses digestive limitations.

Mechanism of Action

Direct delivery to bloodstream ensures maximum cellular availability for energy production, DNA repair, and sirtuin activation.

Molecular Information

663.43 Da
Weight
0
amino acids
Coenzyme / Dinucleotide
Type
Amino Acid Sequence:
Adenine-Ribose-Phosphate-Phosphate-Ribose-Nicotinamide
* Oxidized form (NAD+) of nicotinamide adenine dinucleotide; converts to reduced form (NADH) in metabolic reactions

Pharmacokinetics

Peak
30 min
Half-life
45 min
Cleared
~3.8 hrs
100%50%0%0h6h12h18h24h
Peak
Half-life
Cleared
Various NAD+ infusion studies

Research Indications

Cellular Energy Production

Restores mitochondrial function and ATP synthesis capacity.

Metabolic Optimization

Enhances glucose and fat metabolism efficiency.

Exercise Performance

Improves oxygen utilization and endurance capacity.

Research Protocols

Disclaimer: NAD+ therapy should be administered under medical supervision, especially IV infusions. Start with lower doses to assess tolerance.

GoalDoseFrequencyRoute
General Wellness & Energy100-250mg1-2x weeklyIV or IM
Anti-Aging Protocol250-500mg2-3x weeklyIV infusion
Cognitive Enhancement500-1000mg1-2x weeklyIV infusion
Athletic Performance250-500mg2x weeklyIM injection
Recovery & Repair500-1000mg3x weeklyIV infusion
Maintenance Therapy100-250mg1x weeklySubQ or IM

Timing: Morning administration aligns with natural circadian rhythms and provides sustained energy throughout the day.

Peptide Interactions

How to Reconstitute

Important: Always use bacteriostatic water (BAC). Sterile technique is essential.

1

Clean work surface and wash hands thoroughly

2

Wipe tops of both vials with alcohol swabs

3

Draw appropriate amount of bacteriostatic water (typically 2-3ml for 500mg vial)

4

Inject water slowly into NAD+ vial, aiming at the glass wall

5

Gently swirl (do not shake) until completely dissolved

6

Draw prescribed dose using fresh needle

7

Inject at chosen site (IM: deltoid, glute, or thigh; SubQ: abdomen or thigh)

8

Store remaining solution refrigerated

Dosing Calculator

Calculate your injection volume with visual dosing guide

FINAL CONCENTRATION
2.50mg/mL
Each milliliter contains 2.50 mg of peptide
VISUAL REFERENCE (RESEARCH USE ONLY)

To obtain 250 mcg from this solution:

Draw 0.10 mL=10 units

(1 mL = 100 units on any insulin syringe)

0102030405060708090100
00.10.20.30.40.50.60.70.80.91.0
0.10 mL
10 units

Draw to this mark for 250 mcg

This calculator is for research purposes only. Always verify calculations and consult protocols.

Quality Indicators

Clear, colorless solution

NAD+ solution should be transparent without particles.

Proper refrigeration

Stored consistently at 2-8°C for potency.

Sterile technique

Use proper sterile injection procedures.

Reconstitution timing

Use within 28 days of mixing with bacteriostatic water.

Cloudy or discolored solution

May indicate contamination or degradation.

Room temperature storage

Heat degrades NAD+ rapidly.

What to Expect

  • Week 1-2: Initial energy improvements and mental clarity
  • Week 3-4: Enhanced physical performance and recovery
  • Week 5-8: Sustained energy levels and improved sleep quality
  • Week 9-12: Optimized metabolic function and cognitive benefits

Side Effects & Safety

Side Effects

  • Start with lower doses to assess tolerance
  • IV administration requires medical supervision
  • Monitor for injection site reactions
  • Avoid alcohol consumption during treatment
  • Maintain consistent dosing schedule for best results

When to Stop

  • Severe injection site reactions
  • Signs of allergic reaction
  • Persistent discomfort
  • As directed by healthcare provider

References

3 Studies

Intranasal NAD+ for TBI (2012)

Rats | 20 mg/kg intranasal | Post-TBI treatment | Hippocampal neuroprotection

Demonstrated that intranasal NAD+ delivery protected hippocampal neurons following traumatic brain injury.

Nicotinamide Riboside Safety Study (2019)

Human | 100-1000mg oral daily | 8 weeks | Well-tolerated, increased NAD+

Randomized clinical trial demonstrating long-term NR administration is safe and effectively elevates NAD+ metabolites.

Age-Associated NAD+ Decline (2012)

Human tissue analysis | Multiple organs | 10-50% decline with age

Foundational study showing NAD+ decreases in skin, brain, heart, and liver by 10-50% during adult aging.

Quick Start Guide

Typical Dose
250-500mg
How Often
1-2x weekly
Where to Inject
IM: deltoid
Timing
Morning preferred for energy benefits
Effects Timeline
30 minutes to 2 hours for initial effects, cumulative benefits over weeks
Storage
Refrigerate 2-8°C, use within 28 days after reconstitution
Cycle Length
8-12 weeks with evaluation
Break Between
4-8 weeks between intensive cycles

Research Disclaimer

NAD+ is sold for laboratory research purposes only and is not intended for human or animal consumption. The information provided on this page is compiled from published research, veterinary studies, and anecdotal reports for educational purposes. This content does not constitute medical advice, diagnosis, or treatment recommendations. Any research involving NAD+ must comply with all applicable local, state, and federal regulations. BioInfinity Lab makes no claims regarding the safety or efficacy of NAD+ for any purpose. Consult qualified professionals for any research applications.