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For research and educational purposes only. Not intended for human consumption.

Humanin

Well Researched
Updated Dec 2025

Mitochondrial-Derived Peptide | Neuroprotection & Cytoprotection

Injectable
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Research-only (no established human dose)
Twice weekly (mouse studies)
Injectable
Subcutaneous or IP (research)
Varies by study
Typical duration
2-8°C
Refrigerated

Overview

What is Humanin?

Humanin is a 24-amino-acid mitochondrial-derived peptide with broad cytoprotective effects, including neuroprotection against Alzheimer's disease and age-related cognitive decline. It primarily works through preserving mitochondrial function, inhibiting apoptosis, enhancing mitophagy and biogenesis via PGC-1α upregulation, promoting amyloid-beta phagocytosis, and suppressing inflammation. Variants like HNG show enhanced potency.

Key Benefits

Neuroprotection, anti-apoptotic effects, mitochondrial homeostasis enhancement, cognitive protection, cardioprotection, improved insulin sensitivity.

Mechanism of Action

Enhances mitochondrial biogenesis, mitophagy, electron transport chain activity, and mtDNA expression. Binds/inhibits Bax to prevent mitochondrial outer membrane permeabilization, cytochrome c release, and neuronal death. Attenuates amyloid-beta accumulation and boosts phagocytosis.

Molecular Information

2,687 Da
Weight
24
amino acids
Mitochondrial-derived peptide (MDP)
Type
Amino Acid Sequence:
Met-Ala-Pro-Arg-Gly-Phe-Ser-Cys-Leu-Leu-Leu-Leu-Thr-Ser-Glu-Ile-Asp-Leu-Pro-Val-Lys-Arg-Arg-Ala
* Encoded by mitochondrial genome. HNG variant (S14G substitution) shows superior stability and potency

Pharmacokinetics

Peak
Unknown
Half-life
Limited data
Cleared
Limited data
100%50%0%0h6h12h18h24h
Peak
Half-life
Cleared
Preclinical research

Research Indications

Alzheimer's Disease

Attenuates amyloid-beta accumulation, boosts phagocytosis, and delays cognitive decline in mouse models.

Cognitive Protection

Higher humanin levels correlate with cognitive resilience. Low-humanin SNPs accelerate cognitive aging.

Mitochondrial Function

Preserves and enhances mitochondrial function critical for neuronal health.

Research Protocols

Disclaimer: No human clinical dosing protocols established. Research is preclinical/early-stage. Phase II trials planned for AD, metabolic syndrome, and cardiovascular disease.

GoalDoseFrequencyRoute
Research Protocol (Mouse)Varies by studyTwice weeklyIP or SubQ
HNG Analog (Research)Enhanced potency variantAs per study designIP or SubQ

Timing: No established timing. Research focuses on consistent dosing schedules in animal models.

Peptide Interactions

How to Reconstitute

Important: Always use bacteriostatic water (BAC). Sterile technique is essential.

1

Allow vial to reach room temperature

2

Add appropriate sterile water or BAC water

3

Gently swirl to dissolve - do not shake

4

Solution should be clear

5

Store refrigerated at 2-8°C

6

Use within recommended timeframe per manufacturer

Dosing Calculator

Calculate your injection volume with visual dosing guide

FINAL CONCENTRATION
2.50mg/mL
Each milliliter contains 2.50 mg of peptide
VISUAL REFERENCE (RESEARCH USE ONLY)

To obtain 250 mcg from this solution:

Draw 0.10 mL=10 units

(1 mL = 100 units on any insulin syringe)

0102030405060708090100
00.10.20.30.40.50.60.70.80.91.0
0.10 mL
10 units

Draw to this mark for 250 mcg

This calculator is for research purposes only. Always verify calculations and consult protocols.

Quality Indicators

Limited Human Data

No human clinical trials completed. All dosing is preclinical research-based.

Strong Preclinical Evidence

Extensive research showing neuroprotective and cytoprotective effects in animal models.

Natural Compound

Endogenously produced mitochondrial peptide with physiological relevance.

Analog Variants

HNG (S14G) variant shows superior stability and potency in preclinical models.

Longevity Association

Higher levels associated with centenarian offspring and ethnic groups with lower AD risk.

What to Expect

  • No established human timeline
  • Mouse studies: Twice-weekly HNG injections improved metabolic healthspan
  • Humanin injections delayed age-related cognitive decline in mice
  • Effects may take weeks to months to manifest in animal studies
  • Human trials planned but not yet completed

Side Effects & Safety

Side Effects

  • Limited safety data - primarily preclinical research
  • No serious adverse events reported in animal studies
  • Endogenous peptide with expected physiological safety profile
  • Monitor for any unexpected effects
  • Not approved for human use

When to Stop

  • Any unexpected adverse effects
  • Signs of allergic reaction
  • As directed by research protocol
  • Consult healthcare provider

References

3 Studies

P3S Variant and APOE4 Protection (2024)

Human genetics + Mouse | Various | Resists APOE4 amyloid pathology

P3S variant enriched in Ashkenazi centenarians resists APOE4 amyloid pathology via phagocytosis enhancement.

Cognitive Aging SNP Study (2024)

Human longitudinal | Various | Low-humanin SNPs accelerate cognitive aging

Longitudinal human data showing low-humanin SNPs accelerate cognitive aging.

Lifespan Extension in C. elegans

C. elegans | Overexpression | Extended lifespan via FoxO pathway

Humanin overexpression extends lifespan in C. elegans through daf-16/FoxO-dependent mechanisms.

Quick Start Guide

Typical Dose
No established human dose - research only
How Often
Twice weekly in mouse studies
Where to Inject
IP or SubQ in research
Timing
No established timing protocol
Effects Timeline
Weeks to months in animal studies
Storage
Refrigerate at 2-8°C
Cycle Length
As per research protocol
Break Between
Not established

Research Disclaimer

Humanin is sold for laboratory research purposes only and is not intended for human or animal consumption. The information provided on this page is compiled from published research, veterinary studies, and anecdotal reports for educational purposes. This content does not constitute medical advice, diagnosis, or treatment recommendations. Any research involving Humanin must comply with all applicable local, state, and federal regulations. BioInfinity Lab makes no claims regarding the safety or efficacy of Humanin for any purpose. Consult qualified professionals for any research applications.