Peptide Dosing Protocols for Research
Proper dosing is fundamental to peptide research. This guide covers concentration calculations, administration schedules, loading protocols, and documentation practices for reproducible research outcomes.
Research Use Disclaimer
This guide is intended for laboratory research purposes only. All peptides from BioInfinity Lab are sold strictly for in-vitro research and are not intended for human or veterinary use. Dosing information presented here is derived from published research literature for educational purposes.
Accurate dosing ensures research reproducibility. Whether you're following an established protocol or designing your own study, understanding concentration calculations, half-lives, and administration schedules is essential.
Concentration Calculations
Basic Formula
Concentration (mg/mL) = Peptide Mass (mg) ÷ Water Volume (mL)
Example 1
5mg peptide + 2mL BAC water
= 5 ÷ 2 = 2.5 mg/mL
= 2500 mcg/mL
Example 2
10mg peptide + 2mL BAC water
= 10 ÷ 2 = 5 mg/mL
= 5000 mcg/mL
Calculating Injection Volume
Volume (mL) = Desired Dose (mcg) ÷ Concentration (mcg/mL)
Example: 250mcg Dose
If concentration = 2500 mcg/mL
Volume needed = 250 ÷ 2500 = 0.1 mL = 10 units on insulin syringe
Insulin Syringe Conversion
Standard U-100 insulin syringes: 100 units = 1 mL. So 10 units = 0.1 mL, 25 units = 0.25 mL, etc. This makes small-volume measurements easier.
Administration Frequency & Half-Life
Peptide half-life determines how often administration is needed to maintain consistent levels. Shorter half-lives require more frequent dosing.
| Peptide | Half-Life | Typical Frequency |
|---|---|---|
| BPC-157 | ~4 hours | 1-2x daily |
| TB-500 | ~24 hours | 2x weekly (loading), 1x weekly (maintenance) |
| CJC-1295 (No DAC) | ~30 minutes | 1-3x daily (pre-bed common) |
| Ipamorelin | ~2 hours | 1-3x daily |
| GLP1-S | ~7 days | 1x weekly |
| GHK-Cu | ~30 minutes (IV) | 1x daily (topical varies) |
Timing Considerations
- • GH-releasing peptides: Often dosed pre-bed or fasted for optimal effect
- • BPC-157: Often split into 2 doses (AM/PM) for short half-life coverage
- • GLP1-S/GLP-1s: Same day each week for consistency
Loading vs. Maintenance Phases
Some peptide protocols use a "loading" phase with higher initial doses, then transition to a lower "maintenance" phase. This is most common with peptides that accumulate in tissues over time.
Loading Phase
- • Higher doses or frequency
- • Typically 2-4 weeks
- • Establishes tissue saturation
- • Example: TB-500 at 2x weekly
Maintenance Phase
- • Lower doses or frequency
- • Ongoing (varies by protocol)
- • Maintains established levels
- • Example: TB-500 at 1x weekly
Example: TB-500 Protocol
| Phase | Duration | Dose | Frequency |
|---|---|---|---|
| Loading | Weeks 1-4 | 2-5mg | 2x weekly |
| Maintenance | Weeks 5+ | 2-5mg | 1x weekly or bi-weekly |
Research Documentation Best Practices
Thorough documentation enables reproducibility and troubleshooting. Record these details for each research protocol:
Frequently Asked Questions
How do I calculate peptide concentration?
Divide peptide mass (mg) by water volume (mL). Example: 5mg in 2mL = 2.5mg/mL = 2500mcg/mL.
What is a loading phase?
Higher initial doses or frequency to establish tissue saturation before transitioning to lower maintenance doses. Common with TB-500.
How does half-life affect dosing frequency?
Shorter half-life = more frequent dosing needed. BPC-157 (4hr) needs 1-2x daily; GLP1-S (7 days) needs only weekly.
Should I dose peptides fasted?
GH-releasing peptides (CJC-1295, Ipamorelin) are often dosed fasted or pre-bed for optimal effect. Other peptides vary—check specific protocols.
How do I convert between units and mL?
On U-100 insulin syringes: 100 units = 1mL. So 10 units = 0.1mL, 50 units = 0.5mL.