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For research and educational purposes only. Not intended for human consumption.

GHK-Cu

Well Researched
Updated Dec 2025

Copper Peptide | Skin Regeneration & Anti-Aging Compound

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1-4 mg
1x daily
Injectable
Subcutaneous (abdomen
4-12 weeks
Typical duration
Refrigerated
Refrigerated

Overview

What is GHK-Cu?

GHK-Cu is a naturally occurring copper tripeptide found in human plasma, saliva, and urine. Plasma levels decline from ~200 ng/ml at age 20 to ~80 ng/ml by age 60. Research shows it affects 31.2% of human genes and promotes wound healing, collagen synthesis, and anti-aging effects at the cellular level. Injectable GHK-Cu provides systemic distribution for tissue regeneration research.

Key Benefits

Enhanced collagen production, accelerated wound healing, tissue regeneration, anti-inflammatory effects, hair follicle stimulation, systemic anti-aging research applications.

Mechanism of Action

Injectable GHK-Cu provides systemic copper peptide delivery. Activates fibroblasts, stimulates collagen and elastin synthesis, promotes angiogenesis, modulates over 4,000 genes including those involved in DNA repair, antioxidant responses, and stem cell activity.

Molecular Information

404.96 Da
Weight
3
amino acids
Copper tripeptide
Type
Amino Acid Sequence:
Gly-His-Lys
* Complexed with copper (Cu2+) for enhanced bioavailability

Pharmacokinetics

Peak
30-60 min
Half-life
1-2 hrs
Cleared
4-6 hrs
100%50%0%0h6h12h18h24h
Peak
Half-life
Cleared
PMC4180391

Research Indications

Collagen Synthesis (9-fold increase)

Rat wound studies show subcutaneous GHK-Cu increases total protein and collagen synthesis vs. saline placebo. 9-fold increase in collagen synthesis observed in healthy rats. Stimulates type I and III collagen production.

Angiogenesis & Blood Vessel Growth

Promotes VEGF and bFGF expression for new blood vessel formation. Rabbit wound models showed improved blood vessel growth, granular tissue formation, and antioxidant enzyme activity with GHK-Cu injections.

Extracellular Matrix Remodeling

Enhances ECM production, modulates metalloproteinases and TIMP-1/2 for optimal collagen/glycosaminoglycan turnover. Accelerates wound contraction and epithelialization in wound chamber studies.

Diabetic Wound Healing

Diabetic rat models healed faster with GHK-Cu, showing higher glutathione, ascorbic acid levels, enhanced fibroblast/mast cell activation, and improved epithelialization rates.

Ischemic Wound Recovery

Ischemic rat wounds treated with GHK-Cu showed faster healing, reduced MMP-2/MMP-9 metalloproteinases, and lower TNF-β inflammatory levels.

Research Protocols

Disclaimer: These are commonly discussed research protocols and not medical advice. Consult a healthcare provider before use.

GoalDoseFrequencyRoute
Tissue regeneration1-2 mg1x dailySubcutaneous
Wound healing2-4 mg1x dailySubcutaneous near site
Anti-aging research1-2 mg1x dailySubcutaneous
Hair follicle research1-2 mg1x dailySubcutaneous (scalp area)
Systemic collagen support2-3 mg1x dailySubcutaneous

Timing: Inject GHK-Cu subcutaneously, preferably in the morning or near target tissue for localized effects. Due to short half-life (1-2 hrs), daily dosing is typical. Reconstitute with bacteriostatic water.

Peptide Interactions

How to Reconstitute

Important: Always use bacteriostatic water (BAC). Sterile technique is essential.

1

Clean work area and hands thoroughly

2

Calculate required BAC water volume using calculator below

3

Draw BAC water into syringe

4

Inject slowly down vial side (not directly onto powder)

5

Gently swirl until dissolved (never shake)

6

Store in refrigerator, use within 28 days

Dosing Calculator

Calculate your injection volume with visual dosing guide

FINAL CONCENTRATION
2.50mg/mL
Each milliliter contains 2.50 mg of peptide
VISUAL REFERENCE (RESEARCH USE ONLY)

To obtain 250 mcg from this solution:

Draw 0.10 mL=10 units

(1 mL = 100 units on any insulin syringe)

0102030405060708090100
00.10.20.30.40.50.60.70.80.91.0
0.10 mL
10 units

Draw to this mark for 250 mcg

This calculator is for research purposes only. Always verify calculations and consult protocols.

Quality Indicators

White, Fluffy Cake

Lyophilized powder should appear as a white, fluffy 'cake' that fills most of the vial bottom. This indicates proper freeze-drying.

Light Blue Solution

When properly mixed with BAC water, solution should have a light blue tint from copper content.

Slight Clumping

Small clumps that dissolve completely with gentle swirling are acceptable. Shipping can cause minor compaction.

Collapsed or Melted Appearance

If powder appears collapsed, melted, or stuck to vial sides, it may have been exposed to heat during shipping.

Cloudy After Reconstitution

Persistent cloudiness, particles, or precipitates after gentle mixing indicate degraded or contaminated peptide.

What to Expect

  • Week 1-2: Initial tissue response, reduced inflammation at injection sites
  • Week 2-4: Enhanced wound healing, improved tissue repair processes
  • Week 4-8: Visible tissue regeneration, collagen remodeling begins
  • Week 8-12: Significant tissue improvement, musculoskeletal repair benefits
  • Hair growth (scalp injections): 6-12 weeks for initial results
  • Most effective for: Wound healing, tissue regeneration, musculoskeletal repair, anti-aging

Side Effects & Safety

Side Effects

  • Injection site reactions: mild redness, swelling, or bruising (common, resolves in 24-48 hrs)
  • Characteristic light blue coloration at injection site from copper content
  • Mild warmth or tingling at injection area (normal, indicates absorption)
  • Rare: headache, nausea, or fatigue in first few days (usually temporary)
  • Very rare: allergic reaction - discontinue if hives, difficulty breathing, or severe swelling occur

When to Stop

  • Signs of infection at injection site (increasing redness, pus, fever)
  • Severe allergic reaction (anaphylaxis symptoms)
  • Unusual systemic effects lasting more than 48 hours
  • Persistent pain or swelling beyond normal healing time
  • Consult healthcare provider before starting or if concerns arise

References

17 Studies

GHK-Cu Promotes Mucosal Healing in Ulcerative Colitis

Animal Model | Frontiers in Pharmacology | 2025 | Systemic GHK-Cu | IBD

Systemic GHK-Cu administration promoted mucosal healing in DSS-induced ulcerative colitis by enhancing tight junction proteins (ZO-1, Occludin), increasing goblet cells, and suppressing TNF-α/IL-6/IL-1β via SIRT1/STAT3 pathway.

View Study

Intranasal GHK-Cu Delays Cognitive Impairment in Alzheimer's Model

Animal Model | PMC10690187 | 2023 | 5xFAD Mice | Neuroprotection

8-12 weeks intranasal GHK-Cu in transgenic AD mice reduced amyloid plaques in frontal cortex and hippocampus, lowered MCP-1 neuroinflammation markers, and delayed cognitive impairment approaching non-transgenic control levels.

View Study

GHK-Cu/CaT-ME Accelerates Hair Regrowth via Wnt/β-Catenin

Animal Model | PMC10643103 | 2023 | Hair Cycle Study

GHK-Cu in ionic liquid microemulsion demonstrated faster hair regrowth (6 days vs 8-9 days control) by activating Wnt/β-Catenin pathway, optimizing microcirculation, and stimulating growth factors.

View Study

Quick Start Guide

Typical Dose
1-4 mg
How Often
1x daily
Where to Inject
Subcutaneous (abdomen
Timing
Morning or near target tissue
Effects Timeline
2-4 weeks for tissue effects
Storage
Refrigerated 2-8°C
Cycle Length
4-12 weeks
Break Between
4+ weeks

Research Disclaimer

GHK-Cu is sold for laboratory research purposes only and is not intended for human or animal consumption. The information provided on this page is compiled from published research, veterinary studies, and anecdotal reports for educational purposes. This content does not constitute medical advice, diagnosis, or treatment recommendations. Any research involving GHK-Cu must comply with all applicable local, state, and federal regulations. BioInfinity Lab makes no claims regarding the safety or efficacy of GHK-Cu for any purpose. Consult qualified professionals for any research applications.