For research and educational purposes only. Not intended for human consumption.
Epitalon
Well Researched- •2025 hTERT upregulation study (PMID: 40908429)
- •ALT pathway research in cancer cells
- •Biological age reduction case study (7.9 yrs)
Epithalon | AEDG Tetrapeptide | Telomerase Activator
Overview
What is Epitalon?
Epitalon (Epithalon, AEDG) is a synthetic tetrapeptide consisting of four amino acids (Ala-Glu-Asp-Gly) based on a naturally occurring pineal gland peptide. Developed by Russian scientist Vladimir Khavinson and studied for over 35 years, Epitalon is primarily researched for its ability to activate telomerase, extend telomeres, and potentially slow cellular aging. Recent 2025 research confirms its mechanism via hTERT upregulation, with studies showing dose-dependent telomere extension from 2.4kb to 4kb in human cells.
Key Benefits
Telomerase activation via hTERT upregulation for telomere extension (2.4kb to 4kb in studies), enhanced melatonin production, improved immune function, potential lifespan extension (25-33% in animal models), epigenetic chromatin loosening for enhanced DNA repair, and systemic anti-aging effects through cellular rejuvenation.
Mechanism of Action
Activates telomerase enzyme by upregulating hTERT mRNA at extremely low concentrations, binds promoter regions (ATTTC motifs) to loosen chromatin and enhance DNA repair, stimulates pineal gland for melatonin production, modulates gene expression through epigenetic mechanisms, and enhances antioxidant enzyme systems including BDNF.
Molecular Information
Pharmacokinetics
Research Indications
Telomere Extension
Activates telomerase to lengthen telomeres and extend cellular replicative lifespan at microgram doses.
Cellular Rejuvenation
Reverses markers of cellular aging and improves mitochondrial function through low-dose signaling.
Lifespan Extension
Animal studies show 25-33% lifespan increase using 30-40 μg/kg doses.
Research Protocols
Disclaimer: Epitalon is commonly cycled in 10-20 day periods, followed by 4-6 month breaks. Current Western dosing protocols may use higher doses than original Russian research. This is not medical advice. Consult a healthcare provider before use.
| Goal | Dose | Frequency | Route |
|---|---|---|---|
| Evidence-Based Protocol | 200-500μg | Daily | Subcutaneous |
| Conservative Start | 100μg | Daily | Subcutaneous |
| Russian Clinical Equivalent | 300μg | Daily | Subcutaneous |
| Ultra-Low Dose | 50μg | Daily | Subcutaneous |
Timing: Morning administration aligns with natural cortisol rhythm and may enhance circadian regulation. Some prefer evening dosing for enhanced sleep effects.
Peptide Interactions
How to Reconstitute
Important: Always use bacteriostatic water (BAC). Sterile technique is essential.
Clean vial tops with alcohol swab and allow to dry
For corrected dosing: Add 5ml bacteriostatic water to create appropriate concentration
Inject water slowly into peptide vial, aiming at the side wall
Gently swirl vial - do not shake vigorously to preserve peptide structure
Solution should be clear and colorless when properly mixed
Store reconstituted solution in refrigerator immediately
Dosing Calculator
Calculate your injection volume with visual dosing guide
To obtain 250 mcg from this solution:
Draw 0.10 mL=10 units
(1 mL = 100 units on any insulin syringe)
Draw to this mark for 250 mcg
This calculator is for research purposes only. Always verify calculations and consult protocols.
Quality Indicators
White Lyophilized Powder
Pure Epitalon appears as white to off-white freeze-dried powder.
Clear Solution After Reconstitution
Properly mixed Epitalon should be completely clear and colorless.
Certificate of Analysis Available
Reputable suppliers provide third-party testing showing >98% purity.
Yellow or Discolored Powder
Indicates degradation or impurities - do not use.
Cloudy Solution After Mixing
Suggests contamination or improper storage - discard.
What to Expect
- •Day 1-3: Usually no immediate effects, some report subtle energy improvement
- •Day 3-7: Better sleep quality, more vivid dreams (melatonin effect)
- •Week 2: Enhanced morning energy, improved mood stability
- •Week 3-4: Skin quality improvements, better wound healing
- •Month 2-3: Laboratory markers may show telomere length changes
- •Long-term: Cumulative anti-aging effects with repeated cycles
Side Effects & Safety
Side Effects
- •Generally well-tolerated with minimal side effects in studies
- •May cause mild injection site reactions
- •Some users report vivid dreams due to melatonin enhancement
- •No severe adverse events reported in 30+ years of Russian clinical use
- •Theoretical concern about telomerase in cancer patients - avoid if active malignancy
When to Stop
- •Signs of infection at injection site
- •Allergic reactions
- •Active cancer diagnosis
- •Unusual symptoms
- •Consult healthcare provider
References
6 StudiesTelomerase Activation in Human Fibroblasts
Human cells | 0.1μM | 5 days | Extended Hayflick limit
Landmark study showed Epitalon activated telomerase in telomerase-negative human fibroblasts, extending their replicative lifespan from 34 to 44+ passages while maintaining telomere length comparable to young cells.
View StudyhTERT Upregulation Study (2025)
Human epithelial & fibroblast cells | 0.2-1 μg/ml | 4 days | Dose-dependent telomere extension
Confirmed Epitalon induces telomerase activity by upregulating hTERT mRNA (the catalytic subunit of telomerase), extending telomeres from 2.4kb to 4kb in normal cells (IBR3, HMEC) with elevated telomerase activity.
View StudyALT Pathway in Cancer Cells (2025)
Cancer cell lines (21NT, BT474) | Multiple doses | Cell-type specific mechanism
Demonstrated Epitalon extends telomeres in some cancer cells via Alternative Lengthening of Telomeres (ALT) rather than telomerase, showing important cell-type specificity for safety considerations.
View StudyQuick Start Guide
Research Disclaimer
Epitalon is sold for laboratory research purposes only and is not intended for human or animal consumption. The information provided on this page is compiled from published research, veterinary studies, and anecdotal reports for educational purposes. This content does not constitute medical advice, diagnosis, or treatment recommendations. Any research involving Epitalon must comply with all applicable local, state, and federal regulations. BioInfinity Lab makes no claims regarding the safety or efficacy of Epitalon for any purpose. Consult qualified professionals for any research applications.